PXD012874-2

PXD012874 is an original dataset announced via ProteomeXchange.

Title	Identification of different human CD8 T cell subsets by superior cytotoxicity in human T cells
Description	Cytotoxic CD8+ T cells can effectively kill target cells by producing cytokines, chemokines and granzymes. Expression of these effector molecules is however highly divergent, and tools that identify and pre-select potent killer cells are lacking. Human CD8+ T cells can be divided into IFNGand IL-2 producing cells. Unbiased RNA-sequencing and proteomics analysis on cytokine-producing fixed CD8+ T cells revealed that IL-2+ T cells produce helper cytokines, and that IFNG+ T cells produce cytotoxic molecules. IFNG+ cytotoxic T cells expressed the surface marker CD29 already prior to stimulation. CD29 also marked T cells with cytotoxic gene expression from different tissues in single-cell RNA-sequencing data. Notably, the cytotoxic features of CD29+ T cells were maintained during cell culture, suggesting a stable phenotype. Pre-selecting CD29-expressing MART1 TCR-engineered T cells potentiated the killing of target cells. We therefore propose that selecting for CD29+ T cells could boost the anti-tumoral activity of T cell therapeutics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:25:47.641.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maartje van den Biggelaar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 08:48:08	ID requested
1	2021-09-08 09:06:06	announced
⏵ 2	2024-10-22 05:25:56	announced	2024-10-22: Updated project metadata.

10.1073/pnas.1913940117;

Nicolet BP, Guislain A, van Alphen FPJ, Gomez-Eerland R, Schumacher TNM, van den Biggelaar M, Wolkers MC, T cells with an increased cytotoxic potential. Proc Natl Acad Sci U S A, 117(12):6686-6696(2020) [pubmed]

submitter keyword: Human, IL2, IFNG, CD8, T cells

Maartje van den Biggelaar
contact affiliation	Laboratory of Proteomics, Department of Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam, the Netherlands
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Maartje van den Biggelaar
contact affiliation	Sanquin Research
contact email	m.vandenbiggelaar@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD012874

PRIDE project URI

• PRIDE
  1. PXD012874
     1. Label: PRIDE project
     2. Name: Identification of different human CD8 T cell subsets by superior cytotoxicity in human T cells