PXD012853-1

PXD012853 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription
Description	Sperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional program for embryonic development. We show for the first time that remodelling of histones during spermiogenesis results in the retention of methylated histone H3 at the same genomic location in every sperm cell. This homogeneously methylated fraction of histone H3 in the sperm genome is maintained during early embryonic replication. Such methylated histone fraction resisting postfertilisation reprogramming marks developmental genes whose expression is perturbed upon experimental reduction of histone methylation. A similar homogeneously methylated histone H3 fraction is detected in human sperm. Altogether, we uncover a conserved mechanism of paternal epigenetic information transmission to the embryo through the homogeneous retention of methylated histone in a sperm cells population.
HostingRepository	PRIDE
AnnounceDate	2020-05-29
AnnouncementXML	Submission_2020-05-28_23:42:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	jerome jullien
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 01:19:27	ID requested
⏵ 1	2020-05-28 23:42:21	announced
2	2020-07-18 18:40:16	announced	2020-07-19: Updated publication reference for PubMed record(s): 32661239.
3	2024-10-22 05:06:25	announced	2024-10-22: Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

curator keyword: Biological
submitter keyword: sperm, epigenetics, programming, LC-MS/MS, ice-ChIP

curator keyword: Biological

submitter keyword: sperm, epigenetics, programming, LC-MS/MS, ice-ChIP

Contact List

jerome jullien
contact affiliation	gurdon institute
contact email	j.jullien256@gmail.com
lab head
jerome jullien
contact affiliation	gurdon institute
contact email	j.jullien256@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD012853
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD012853

PRIDE project URI

Repository Record List

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