PXD012836 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Approaching absolute quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29 |
Description | Protein extracts of three yeast strains (Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS6556 and Yarrowia lipolytica W29) cultivated in chemostats under different conditions. Representative samples containing aliquots of all conditions for each yeast strain were spiked with UPS2 standard (Sigma) to estimate absolute values in fmol. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: - Standard condition : 30°C, pH 5.5 - High temperature: 36°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress : 30°C, pH 5.5, 1M KCl The conditions for Kluyveromyces marxianus CBS6556 are: - Standard condition : 30°C, pH 5.5 - High temperature: 40°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress: 30°C, pH 5.5, 0.6 M KCl The conditions for Yarrowia lipolytica W29 are: - Standard condition: 28°C, pH 5.5 - High temperature: 32°C, pH 5.5 - Low pH: 28°C, pH 3.5 This study is part of the OMICS data generation WP of CHASSY project (European Union’s Horizon 2020 grant agreement No 720824). |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-15 |
AnnouncementXML | Submission_2022-02-15_09:11:20.157.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aaron Millan Oropeza |
SpeciesList | scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Kluyveromyces marxianus DMKU3-1042; NCBI TaxID: 1003335; |
ModificationList | alkylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-22 03:40:56 | ID requested | |
⏵ 1 | 2022-02-15 09:11:21 | announced | |
Publication List
Mill, á, n-Oropeza A, Blein-Nicolas M, Monnet V, Zivy M, Henry C, Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes, 10(1):(2022) [pubmed] |
Keyword List
submitter keyword: yeast, LC-MS/MS, shotgun, systems biology, UPS2 |
Contact List
Véronique Monnet |
contact affiliation | Micalis Institute, INRA, PAPPSO, Université Paris-Saclay, Jouy-en-Josas, France. |
contact email | veronique.monnet@inra.fr |
lab head | |
Aaron Millan Oropeza |
contact affiliation | INRA |
contact email | aaron.millan-oropeza@inra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012836
- Label: PRIDE project
- Name: Approaching absolute quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29