PXD012806-2

PXD012806 is an original dataset announced via ProteomeXchange.

Title	Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae
Description	Introduced for mass immunization in the 1920s, vaccines against diphtheria are among the oldest and safest vaccines known. The basic principle of their production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins may be secreted by Corynebacterium diphtheriae during cultivation, we assumed that diphtheria toxoid might not be the only component present in the vaccine. In this study, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Western blot analyses indicated that at least some of these are immunogenic and may therefore support protection against C. diphtheriae. In frame of this study, we could show that the C. diphtheriae toxoid vaccines also induce antibodies directed against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen which is outnumbering C. diphtheriae as cause of diphtheria-like illness in Western Europe, but do not induce immune reaction against other C. ulcerans proteins.
HostingRepository	PRIDE
AnnounceDate	2019-04-18
AnnouncementXML	Submission_2019-05-03_09:05:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Möller
SpeciesList	scientific name: Corynebacterium diphtheriae NCTC 13129; NCBI TaxID: 257309; scientific name: Clostridium tetani E88; NCBI TaxID: 212717;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-20 09:30:35	ID requested
1	2019-04-18 03:23:59	announced
⏵ 2	2019-05-03 09:05:27	announced	Updated publication reference for PubMed record(s): 31036455.
3	2024-10-22 04:53:25	announced	2024-10-22: Updated project metadata.

M, ö, ller J, Kraner M, Sonnewald U, Sangal V, Tittlbach H, Winkler J, Winkler TH, Melnikov V, Lang R, Sing A, Mattos-Guaraldi AL, Burkovski A, Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae. Vaccine, 37(23):3061-3070(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: coryebacteria, exoproteome, mass spectrometry, proteomics, secretome, vaccination

Andreas Burkovski
contact affiliation	Department Biologie Professur für Mikrobiologie
contact email	andreas.burkovski@fau.de
lab head
Jens Möller
contact affiliation	FAU Erlangen
contact email	Jens.moeller@fau.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012806
     1. Label: PRIDE project
     2. Name: Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae