PXD012800 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | On-chip AP-MS: Interaction proteomics on a microfluidics platform |
Description | Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin, CCC and Mediator complex from as little as 4 micrograms of input lysate, representing a 50─100-fold downscaling compared to regular microcentrifuge tube-based protocols. We show that our platform can be used to affinity purify tagged baits as well as native cellular proteins and their interaction partners. As such, our method holds great promise for future biological and clinical AP-MS applications in which sample amounts are limited. |
HostingRepository | PRIDE |
AnnounceDate | 2019-04-02 |
AnnouncementXML | Submission_2019-04-02_08:28:10.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cristina Furlan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-20 06:46:23 | ID requested | |
⏵ 1 | 2019-04-02 08:28:11 | announced | |
2 | 2019-05-14 07:19:17 | announced | Updated publication reference for PubMed record(s): 30948724. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: AP-MS, low input, interaction proteomics |
Contact List
Michiel Vermeulen |
contact affiliation | Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands |
contact email | Michiel.Vermeulen@science.ru.nl |
lab head | |
Cristina Furlan |
contact affiliation | Radboud University |
contact email | c.furlan@science.ru.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012800 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012800
- Label: PRIDE project
- Name: On-chip AP-MS: Interaction proteomics on a microfluidics platform