PXD012799 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An Archaeal Pilus Survives the Most Extreme Conditions Through Extensive Glycosylation |
Description | Pili on the surface of Sulfolobus islandicus are used for a host of functions, and serve as receptors for certain archaeal viruses. We find that these pili, when removed from cells, resist digestion by trypsin or pepsin, and survive boiling in SDS or 5M guanidinium-HCl. We have used cryo-EM to determine the structure of these filaments at 4.1 Å resolution. An atomic model was built by combining the map with bioinformatics without prior knowledge of the pilin sequence, an approach that should prove useful when looking at assemblies where all of the components may not be known. The atomic structure of the archaeal pilus was unusual due to almost a third of the residues being either threonine or serine and many hydrophobic surface residues. While the map showed specific glycosylation of only three residues, mass per unit length measurements suggested extensive glycosylation. We show that this extensive glycosylation renders these filaments soluble and provides the remarkable structural stability. We also show that the overall fold of the archaeal pilin is quite similar to archaeal flagellin, establishing common evolutionary origins. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-26 |
AnnouncementXML | Submission_2020-05-26_13:24:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JJ Park |
SpeciesList | scientific name: Sulfolobus islandicus LAL14/1; NCBI TaxID: 1241935; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-20 06:45:29 | ID requested | |
⏵ 1 | 2020-05-26 13:24:23 | announced | |
Publication List
Wang F, Cvirkaite-Krupovic V, Kreutzberger MAB, Su Z, de Oliveira GAP, Osinski T, Sherman N, DiMaio F, Wall JS, Prangishvili D, Krupovic M, Egelman EH, An extensively glycosylated archaeal pilus survives extreme conditions. Nat Microbiol, 4(8):1401-1410(2019) [pubmed] |
Keyword List
submitter keyword: Pili, Cryo-EM, glycosylation, LC-MS |
Contact List
Edward H. Egelman |
contact affiliation | Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA |
contact email | egelman@virginia.edu |
lab head | |
JJ Park |
contact affiliation | University of Virginia |
contact email | jp2ht@virginia.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012799
- Label: PRIDE project
- Name: An Archaeal Pilus Survives the Most Extreme Conditions Through Extensive Glycosylation