PXD012766-2

PXD012766 is an original dataset announced via ProteomeXchange.

Title	Utilisation of Galleria mellonella larvae to characterise development of Staphylococcus aureus infection
Description	Staphylococcus aureus is a common human opportunistic pathogen which causes a wide range of infections from superficial skin infections to invasive infections including pneumonia and bloodstream infections. Here we describe how an inoculum of S. aureus activates the cellular and humoral response of Galleria mellonella larvae while growing and disseminating throughout the host, forming nodules and ultimately killing the host. An inoculum of S. aureus (2×106/ larva) decreased larval viability at 24 (80 ± 5.77%), 48 (55.93 ± 5.55%) and 72 (10.23 ± 2.97%) hours and this was accompanied by significant proliferation and disseminated of S. aureus between 6 hours to 48 hours and the formation of nodules in the host. The hemocyte (immune cell) densities increased between 4 and 24 hours and cells isolated from larvae after 6 and 24 hours exposure to heat killed S. aureus (2×106/ larva) showed altered killing kinetics as compared to control larvae. Alterations in the humoral immune response of larvae 6 and 24 hours post infection was also determined by quantitative shotgun proteomics. The proteome of 6 hour infected larvae was enriched for antimicrobial proteins, members of the prophenoloxidase cascade and a range of peptidoglycan recognition proteins. By 24 hours there was a significant increase in a range of antimicrobial peptides with anti-staphylococcal activity and proteins associated with nodule formation. The results presented here find S. aureus interacts with the larval host immune response, induces its expression and also forms nodules which are a hallmark of soft tissue infections during human infection.
HostingRepository	PRIDE
AnnounceDate	2019-05-21
AnnouncementXML	Submission_2019-06-14_02:39:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerard Sheehan
SpeciesList	scientific name: Galleria mellonella; NCBI TaxID: 7137;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-18 07:30:23	ID requested
1	2019-05-21 00:28:49	announced
⏵ 2	2019-06-14 02:39:55	announced	Updated publication reference for PubMed record(s): 31107207.
3	2024-10-22 04:53:30	announced	2024-10-22: Updated project metadata.

Sheehan G, Dixon A, Kavanagh K, Utilization of Galleria mellonella larvae to characterize the development of Staphylococcus aureus infection. Microbiology (Reading), 165(8):863-875(2019) [pubmed]

submitter keyword: : Galleria, Infection, Immunity, Mini-model, Staphylococcus

Kevin Kavanagh
contact affiliation	Department of Biology, Maynooth University, Co. Kildare, Ireland.
contact email	kevin.kavanagh@mu.ie
lab head
Gerard Sheehan
contact affiliation	Maynooth University
contact email	gerard.sheehan.2013@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012766

PRIDE project URI

• PRIDE
  1. PXD012766
     1. Label: PRIDE project
     2. Name: Utilisation of Galleria mellonella larvae to characterise development of Staphylococcus aureus infection