PXD012732-2

PXD012732 is an original dataset announced via ProteomeXchange.

Title	CDC14B interactome - The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).
Description	The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:51.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Gloeckner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-15 05:08:56	ID requested
1	2020-03-25 02:23:54	announced
⏵ 2	2023-11-14 08:49:53	announced	2023-11-14: Updated project metadata.

Dietachmayr M, Rathakrishnan A, Karpiuk O, von Zweydorf F, Engleitner T, Fern, á, ndez-S, á, iz V, Schenk P, Ueffing M, Rad R, Eilers M, Gloeckner CJ, Clemm von Hohenberg K, Bassermann F, Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival. Nat Commun, 11(1):1268(2020) [pubmed]

curator keyword: Biological

submitter keyword: USP9X,CDC14B

Christian Johannes Gloeckner
contact affiliation	1) German Center for Neurodegenerative Diseases (DZNE), 72076 Tübingen, Germany (2) University of Tübingen, Center for Ophthalmology, Institute for Ophthalmic Research, 72074 Tübingen, Germany
contact email	johannes.gloeckner@dzne.de
lab head
Johannes Gloeckner
contact affiliation	DZNE
contact email	johannes.gloeckner@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012732

PRIDE project URI

• PRIDE
  1. PXD012732
     1. Label: PRIDE project
     2. Name: CDC14B interactome - The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).