PXD012693 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pax3 interactome in mouse ES cell-derived mesodermal precursors |
Description | The skeletal myogenic lineage in developing embryos is specified within the somites and requires members of the Paired box (Pax) family of transcription factors. In particular, Pax3 is the first member of this family expressed in the central region of the dermomyotome and it is necessary for specification, migration and survival of the myogenic progenitors that will form the muscles of the limbs, diaphragm and tongue. To investigate Pax3 molecular functions in the developing mesoderm, we generated doxycycline-inducible mouse embryonic stem (ES) cells expressing untagged and HaFlag-tagged-Pax3. These cell lines were used to unbiasedly identify its interacting partners by tandem affinity purification followed by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2019-04-18 |
AnnouncementXML | Submission_2019-05-31_06:08:48.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alessandro Magli |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-13 06:41:14 | ID requested | |
1 | 2019-04-18 07:47:21 | announced | |
⏵ 2 | 2019-05-31 06:08:49 | announced | Updated publication reference for PubMed record(s): 31127120. |
Publication List
Magli A, Baik J, Pota P, Cordero CO, Kwak IY, Garry DJ, Love PE, Dynlacht BD, Perlingeiro RCR, Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification. Nat Commun, 10(1):2316(2019) [pubmed] |
Keyword List
submitter keyword: Pax3, mesoderm, LC-MSMS, embryoid bodies, mouse, myogenesis, affinity purification, nuclear extract |
Contact List
Rita CR Perlingeiro |
contact affiliation | Department of Medicine, Cardiology division, Lillehei Heart Institute, University of Minnesota, USA |
contact email | perli032@umn.edu |
lab head | |
Alessandro Magli |
contact affiliation | University of Minnesota Dept of Medicine Cardiology division Twin Cities campus Minneapolis |
contact email | alemagli@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012693 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012693
- Label: PRIDE project
- Name: Pax3 interactome in mouse ES cell-derived mesodermal precursors