PXD012682-1

PXD012682 is an original dataset announced via ProteomeXchange.

Title	Cleavable Hydrophobic Derivatization Strategy for Enrichment and Identification of Phosphorylated Lysine Peptides
Description	Herein, a cleavable hydrophobic derivatization (CHD) strategy was established for the enrichment and identification of pLys peptides. By CHD, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate (Dec-disulf-NHS) was synthesized to react with lysine dephosphorylated peptides, and then the derived peptides were captured by a C18 column, followed by the cleavage of hydrophobic chain to leave the specific label on the target peptides.
HostingRepository	iProX
AnnounceDate	2019-02-12
AnnouncementXML	Submission_2019-02-12_19:03:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yechen Hu
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-12 18:58:59	ID requested
⏵ 1	2019-02-12 19:03:19	announced
2	2019-12-10 22:08:43	announced	2019-12-11: Update publication information.

Dataset with its publication pending

submitter keyword: Phosphorylated Lysine peptides, Hydrophobic Derivatization, N-phosphorylation, Proteomics, LC-MS/MS

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
Yechen Hu
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	yechenhu@dicp.ac.cn
dataset submitter

iProX dataset URI