PXD012664-1

PXD012664 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Integrative proteomic and phosphoproteomics analysis of human ischemic and dilated cardiomyopathic explant tissues identifies etiology-specific phosphorylation patterns and signaling pathways
Description	Myocardial tissue samples were explanted from the explanted left ventricle of heart failure patients with DCM (n=4), left ventricular infarct, peri-, and non-infarct regions of HF patients with ICM (n=4) at the time of their heart transplant surgeries, and the left ventricle of age- and sex-matched non-failing controls (NFC, n=4). Comparative quantitative analysis was performed on three independent sets (DCM vs. NFC, infarct vs. non-infarct, and peri- vs. non-infarct) labeled with 10-plex tandem mass tags. Following enrichment of phosphorylated peptides, the flow-through and eluted fractions were collected separately and subjected to LC-MS/MS (liquid chromatography-tandem mass spectrometry) on a Q-Exactive HF for global proteomics and phosphoproteomics profiling respectively.
HostingRepository	PRIDE
AnnounceDate	2023-11-20
AnnouncementXML	Submission_2023-11-20_11:48:01.366.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uros Kuzmanov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-11 03:06:03	ID requested
⏵ 1	2023-11-20 11:48:02	announced
2	2024-10-22 06:15:16	announced	2024-10-22: Updated project metadata.

Publication List

Reitz CJ, Tavassoli M, Kim DH, Shah S, Lakin R, Teng ACT, Zhou YQ, Li W, Hadipour-Lakmehsari S, Backx PH, Emili A, Oudit GY, Kuzmanov U, Gramolini AO, Proteomics and phosphoproteomics of failing human left ventricle identifies dilated cardiomyopathy-associated phosphorylation of CTNNA3. Proc Natl Acad Sci U S A, 120(19):e2212118120(2023) [pubmed]
10.1073/pnas.2212118120;

Reitz CJ, Tavassoli M, Kim DH, Shah S, Lakin R, Teng ACT, Zhou YQ, Li W, Hadipour-Lakmehsari S, Backx PH, Emili A, Oudit GY, Kuzmanov U, Gramolini AO, Proteomics and phosphoproteomics of failing human left ventricle identifies dilated cardiomyopathy-associated phosphorylation of CTNNA3. Proc Natl Acad Sci U S A, 120(19):e2212118120(2023) [pubmed]

10.1073/pnas.2212118120;

Keyword List

curator keyword: Biomedical
submitter keyword: heart, phosphoproteomic, cardiac tissue, signaling,QE HF

curator keyword: Biomedical

submitter keyword: heart, phosphoproteomic, cardiac tissue, signaling,QE HF

Contact List

Anthony Orlando Gramolini
contact affiliation	Department of Physiology, University of Toronto Ted Rogers Centre for Heart Research Toronto, Ontario, Canada
contact email	anthony.gramolini@utoronto.ca
lab head
Uros Kuzmanov
contact affiliation	University of Toronto
contact email	uros.kuzmanov@utoronto.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD012664
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD012664

PRIDE project URI

Repository Record List

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