PXD012627-2

PXD012627 is an original dataset announced via ProteomeXchange.

Title	Reductional meiosis I chromosome segregation is established by coordination of key meiotic kinases
Description	Meiosis produces gametes through a specialised, two-step cell division, which is highly error-prone in humans. Reductional meiosis I, where maternal and paternal chromosomes (homologs) segregate, is followed by equational meiosis II, where sister chromatids separate. Uniquely during meiosis I, sister kinetochores are monooriented and pericentromeric cohesin is protected. Here, we demonstrate that these key adaptations for reductional chromosome segregation are achieved through separable control of multiple kinases by the meiosis I-specific budding yeast Spo13 protein. Recruitment of Polo kinase to kinetochores directs monoorientation, while, independently, cohesin protection is achieved by controlling the effects of cohesin kinases. Therefore, reductional chromosome segregation, the defining feature of meiosis, is established by multifaceted kinase control by a master regulator. The recent identification of Spo13 orthologs, fission yeast Moa1 and mouse MEIKIN, suggests that kinase coordination by a master meiosis I regulator may be a general feature in the establishment of reductional chromosome segregation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:56.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adele Marston
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 01:32:32	ID requested
1	2019-11-12 18:42:39	announced
⏵ 2	2024-10-22 04:53:00	announced	2024-10-22: Updated project metadata.

10.1016/j.devcel.2019.04.003;

Galander S, Barton RE, Borek WE, Spanos C, Kelly DA, Robertson D, Rappsilber J, Marston AL, Reductional Meiosis I Chromosome Segregation Is Established by Coordination of Key Meiotic Kinases. Dev Cell, 49(4):526-541.e5(2019) [pubmed]

curator keyword: Biological

submitter keyword: chromosome segregation, LC-MS, kinases,Meiosis

Adele Marston
contact affiliation	WTCCB, University of Edinburgh, Edinburgh, UK
contact email	adele.marston@ed.ac.uk
lab head
Adele Marston
contact affiliation	University of Edinburgh
contact email	adele.marston@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012627

PRIDE project URI

• PRIDE
  1. PXD012627
     1. Label: PRIDE project
     2. Name: Reductional meiosis I chromosome segregation is established by coordination of key meiotic kinases