PXD012624-2

PXD012624 is an original dataset announced via ProteomeXchange.

Title	Reconstitution of microtubule nucleation in vitro reveals novel roles for Mzt1
Description	Protein-protein interaction within the MGM holocomplex has been investigated by chemical cross-linking mass spectrometry using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) cross-linker. Although this analysis was not exhaustive, we observed crosslinks between Alp4 and Alp6 along the length of these two proteins, consistent with their general parallel lateral alignment in current models for gamma-TuC organization. In addition, we observed specific cross-links from both Alp4 and Alp6 to the Mto1 [bonsai] CM1 domain and/or its immediate flanking regions. Interestingly, crosslinks from Alp4 and Alp6 N-terminal regions tended to be to the C-terminal portion of the CM1 domain, while crosslinks from Alp4 and Alp6 C-terminal regions tended to be to the N-terminal portion of the CM1 domain. This raises the possibility that the CM1 domain, which is adjacent to coiled-coil regions, may be oriented antiparallel to Alp4 and Alp6.
HostingRepository	PRIDE
AnnounceDate	2019-05-27
AnnouncementXML	Submission_2019-07-15_01:11:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 01:12:10	ID requested
1	2019-05-27 03:32:30	announced
⏵ 2	2019-07-15 01:11:45	announced	Updated publication reference for PubMed record(s): 31287970.
3	2024-10-22 04:52:57	announced	2024-10-22: Updated project metadata.

Leong SL, Lynch EM, Zou J, Tay YD, Borek WE, Tuijtel MW, Rappsilber J, Sawin KE, Vitro Reveals Novel Roles for Mzt1. Curr Biol, 29(13):2199-2207.e10(2019) [pubmed]

curator keyword: Biological

submitter keyword: “MGM” (Mto/Gamma/Mozart) holocomplex, Cross-linking

Juri Rappsilber
contact affiliation	1Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh 2Chair of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, Berlin, 13355, Germany
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012624

PRIDE project URI

• PRIDE
  1. PXD012624
     1. Label: PRIDE project
     2. Name: Reconstitution of microtubule nucleation in vitro reveals novel roles for Mzt1