PXD012610 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | miR-17-92/106b regulate beta cell mitotic checkpoint and insulin secretion |
Description | Adult beta cells in the pancreas are the sole source of insulin in our body. Beta cell loss or increased demand for insulin, impose metabolic challenges because adult beta cells are generally quiescent and infrequently re-enter the cell division cycle. miR-17-92/106b is a family of proto-oncogene microRNAs, that regulate proliferation in normal tissues and in cancer. Here, we employ mouse genetics to demonstrate a critical role for miR-17-92/106b in glucose homeostasis and in controlling insulin secretion. Mass spectrometry analysis was performed on miR-17-92LoxP/LoxP;106-25-/- MEF lysate, without or with CRE-Adenovirus. miR-17-92LoxP/LoxP;106-25+/+ MEFs with GFP-Adenovirus served as controls. We demonstrate that miR-17-92/106b regulate the adult beta cell mitotic checkpoint and that miR-17-92/106b deficiency results in reduction in beta cell mass in-vivo. Furthermore, protein kinase A (PKA) is a new relevant molecular pathway downstream of miR-17-92/106b in control of adult beta cell division and glucose homeostasis. Therefore, contributes to the understanding of proto-oncogene miRNAs in the normal, untransformed endocrine pancreas, and illustrates new genetic means for regulation of beta cell mitosis and function by non-coding RNAs. |
HostingRepository | PRIDE |
AnnounceDate | 2019-06-17 |
AnnouncementXML | Submission_2019-06-17_05:39:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alon Savidor |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-07 03:43:40 | ID requested | |
⏵ 1 | 2019-06-17 05:39:17 | announced | |
Publication List
Mandelbaum AD, Kredo-Russo S, Aronowitz D, Myers N, Yanowski E, Klochendler A, Swisa A, Dor Y, Hornstein E, miR-17-92 and miR-106b-25 clusters regulate beta cell mitotic checkpoint and insulin secretion in mice. Diabetologia, 62(9):1653-1666(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: LC-MSMS, Mouse, miR-17-92/106b |
Contact List
Eran Hornstein |
contact affiliation | Department of Molecular Genetics Faculty of Biochemistry Weizmann Institute of Science |
contact email | Eran.Hornstein@weizmann.ac.il |
lab head | |
Alon Savidor |
contact affiliation | The Weizmann Institute of Science |
contact email | alon.savidor@weizmann.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012610
- Label: PRIDE project
- Name: miR-17-92/106b regulate beta cell mitotic checkpoint and insulin secretion