PXD012593-4

PXD012593 is an original dataset announced via ProteomeXchange.

Title	TranslationalLandscape_HumanHeart
Description	Here, we analyze the expression of predicted microproteins in human heart tissue and iPSC cardiomyocytes, using shot-gun proteomics and SRM. 4 identified candidates were functionally validated by interactome studies using AP-MS (affinity purification coupled to mass spectrometry). In addition, the impact of disease-causing protein-truncating variants on translation was studied in rat heart tissue by performing shot-gun proteomics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:05.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marieluise Kirchner
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TSQ Quantiva; Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-01 07:40:18	ID requested
1	2019-06-04 03:25:09	announced
2	2019-06-04 06:11:25	announced	Updated publication reference for DOI(s): 10.1016/j.cell.2019.05.010.
3	2019-06-14 02:42:50	announced	Updated publication reference for DOI(s): 10.1016/j.cell.2019.05.010.
⏵ 4	2023-11-14 08:50:06	announced	2023-11-14: Updated project metadata.

van Heesch S, Witte F, Schneider-Lunitz V, Schulz JF, Adami E, Faber AB, Kirchner M, Maatz H, Blachut S, Sandmann CL, Kanda M, Worth CL, Schafer S, Calviello L, Merriott R, Patone G, Hummel O, Wyler E, Obermayer B, M, ü, cke MB, Lindberg EL, Trnka F, Memczak S, Schilling M, Felkin LE, Barton PJR, Quaife NM, Vanezis K, Diecke S, Mukai M, Mah N, Oh SJ, Kurtz A, Schramm C, Schwinge D, Sebode M, Harakalova M, Asselbergs FW, Vink A, de Weger RA, Viswanathan S, Widjaja AA, G, ä, rtner-Rommel A, Milting H, Dos Remedios C, Knosalla C, Mertins P, Landthaler M, Vingron M, Linke WA, Seidman JG, Seidman CE, Rajewsky N, Ohler U, Cook SA, Hubner N, The Translational Landscape of the Human Heart. Cell, 178(1):242-260.e29(2019) [pubmed]

curator keyword: Biological

submitter keyword: shot gun,heart tissue, targeted proteomics

Norbert Hubner
contact affiliation	Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany
contact email	nhuebner@mdc-berlin.de
lab head
Marieluise Kirchner
contact affiliation	Proteomics Platform, BIH@Charite
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012593

PRIDE project URI

• PRIDE
  1. PXD012593
     1. Label: PRIDE project
     2. Name: TranslationalLandscape_HumanHeart