PXD012586-2

PXD012586 is an original dataset announced via ProteomeXchange.

Title	Temporal changes in the wheat leaf apoplastic proteome infected by an incompatible or compatible race of leaf rust (Puccinia triticina)
Description	Wheat leaf rust caused by the pathogenic fungus Puccinia triticina, is a serious threat to bread wheat and durum production in many areas of the world. This plant-pathogen interaction has been studied extensively at the molecular genetics level, however proteomics data are still relatively scarce. The present study investigated temporal changes in the abundance of the apoplastic fluid proteome of wheat leaves infected with either virulent or avirulent races of P. triticina, using a label-free LC-MS-based approach. In general, there was very little difference between inoculated and control apoplastic proteomes as a result of either race of pathogen, until haustoria had become well established in the incompatible interaction, although the resistant host responds to pathogen challenge sooner than the susceptible. In the earlier samplings (up to 72 h after inoculation), there were 46 host proteins with significantly changing abundance, however pathogen proteins were detected only rarely and not reproducibly. This is consistent with the hemi-biotrophic life-style of P. triticina; the pathogen infection is characterized by an early phase of a symbiosis-like relationship, followed by a later stage of pathogenesis, with tissue damage and parasitism. The majority of the host proteins with altered abundance up to 72 h post-inoculation were pathogen-response-related, including peroxidases, chitinases, β-1-3-endo-glucanases and PR-proteins. Five days after inoculation with the incompatible race it was possible to detect 150 PT proteins and 117 host proteins which had significantly increased in abundance as well as 33 host proteins which had significantly decreased in abundance and which represent potential targets of pathogen effectors. The pathogen-expressed proteins – seen most abundantly in the incompatible interaction – were mostly uncharacterized proteins, however many of their functions could be inferred through homology-matching with pBLAST. Pathogen proteins also included several candidate effector proteins, some novel and some which have been reported previously from the purified haustoria proteome.
HostingRepository	PRIDE
AnnounceDate	2019-09-13
AnnouncementXML	Submission_2019-11-12_23:38:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Rampitsch
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Puccinia triticina; NCBI TaxID: 208348;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-01 02:13:07	ID requested
1	2019-09-13 11:37:31	announced
⏵ 2	2019-11-12 23:38:35	announced	2019-11-13: Updated publication reference for PubMed record(s): 31708941.
3	2024-10-22 04:52:55	announced	2024-10-22: Updated project metadata.

Rampitsch C, Huang M, Djuric-Cignaovic S, Wang X, Fernando U, ). Front Plant Sci, 10():1291(2019) [pubmed]

submitter keyword: leaf rust, yellow rust, apoplastic fluid, effector proteins, proteomics

Christof Rampitsch
contact affiliation	Agriculture and Agrifood Canada Morden Research and Development Centre
contact email	chris.rampitsch@canada.ca
lab head
Christof Rampitsch
contact affiliation	Molecular Genetics
contact email	chris.rampitsch@agr.gc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012586
     1. Label: PRIDE project
     2. Name: Temporal changes in the wheat leaf apoplastic proteome infected by an incompatible or compatible race of leaf rust (Puccinia triticina)