PXD012579-1

PXD012579 is an original dataset announced via ProteomeXchange.

Title	Stk40 deletion enhances c-Jun protein stability and compromises mesoderm differentiation
Description	Serine/threonine kinase 40 (Stk40) was previously identified as a direct target gene of pluripotency-associated transcription factor Oct4 and its overexpression could facilitate differentiation of mouse embryonic stem cells (mESCs) towards the extraembryonic endoderm. Stk40-/- mice are lethal at the perinatal stage, displaying multiple organ failures. However, the molecular mechanisms underlying the physiological functions of Stk40 remain elusive. Here, we report that Stk40 ablation compromises the mesoderm differentiation from mESCs in vitro and in embryos. Mechanistically, Stk40 interacts with both mammalian constitutive photomorphogenic protein 1 (Cop1) and c-Jun, promoting degradation of c-Jun. Consequently, Stk40 knockout leads to c-Jun protein accumulation, which, in turn, might suppress the Wnt signaling activity and impair the mesoderm differentiation process. Overall, this study reveals that Stk40, together with Cop1, represent a novel axis for modulating c-Jun protein levels within an appropriate range during mesoderm differentiation from mESCs. Our finding provides new insight into the molecular mechanism regulating c-Jun protein stability and may have potential for managing related cellular disorders.
HostingRepository	PRIDE
AnnounceDate	2019-05-14
AnnouncementXML	Submission_2019-05-14_02:52:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaofei Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-31 23:46:10	ID requested
⏵ 1	2019-05-14 02:52:06	announced
2	2024-10-22 04:52:39	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Mouse, MEFs,

Ying Jin
contact affiliation	Shanghai Key Laboratory of Reproductive Medicine，Basic Clinical Research Center, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 227 South Chongqing Road, Shanghai, 200025, China School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai 201210, China
contact email	yjin@sibs.ac.cn
lab head
Xiaofei Liu
contact affiliation	Nanjing Medical University
contact email	1215224830@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012579

PRIDE project URI

• PRIDE
  1. PXD012579
     1. Label: PRIDE project
     2. Name: Stk40 deletion enhances c-Jun protein stability and compromises mesoderm differentiation