PXD012577-2

PXD012577 is an original dataset announced via ProteomeXchange.

Title	Time-dependent analysis of Paenarthrobacter nicotinovorans pAO1 nicotine-related proteome
Description	We used nanoLC–MS/MS in a shotgun proteomics approach in order to identify the proteins expressed by P. nicotinovorans in the presence of nicotine at 3 different time intervals: 7, 10 and 24 hours post inoculation. A total of 915 proteins grouped 584 non-redundant clusters in were identified with an FDR of 0.3% when P. nicotinovorans was grown in on citrate medium alone and on citrate supplemented 0.05% nicotine. The differences in protein expression pattens at different time intervals allows us have a more dynamic picture on how the nicotine catabolic pathway is activated and to related it to the appearance of the known metabolic intermediates.
HostingRepository	PRIDE
AnnounceDate	2021-06-01
AnnouncementXML	Submission_2021-06-15_22:27:26.884.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012577
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marius Mihasan
SpeciesList	scientific name: Paenarthrobacter nicotinovorans; NCBI TaxID: 29320;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Xevo G2 Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-31 23:25:07	ID requested
1	2021-06-01 07:08:13	announced
⏵ 2	2021-06-15 22:27:27	announced	2021-06-16: Updated publication reference for PubMed record(s): 34124447.

Mih, ă, ş, an M, Boiangiu R Ş, Guzun D, Babii C, Aslebagh R, Channaveerappa D, Dupree E, Darie CC, pAO1 Nicotine-Related Proteome. ACS Omega, 6(22):14242-14251(2021) [pubmed]

curator keyword: Biological

submitter keyword: Paenarthrobacter nicotinovorans, nicotine, metabolism, citrate medium

Costel C. Darie
contact affiliation	Biochemistry & Proteomics Group Department of Chemistry & Biomolecular Science, Clarkson University 8 Clarkson Avenue, Potsdam, NY, 13699-5810, USA Office: (315) 268-7763; Lab: (315) 268-2348; Fax: (315) 268-6610
contact email	cdarie@clarkson.edu
lab head
Marius Mihasan
contact affiliation	Alexandru Ioan Cuza University of Iasi
contact email	marius.mihasan@uaic.ro
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD012577

PRIDE project URI

• PRIDE
  1. PXD012577
     1. Label: PRIDE project
     2. Name: Time-dependent analysis of Paenarthrobacter nicotinovorans pAO1 nicotine-related proteome