PXD012564-3

PXD012564 is an original dataset announced via ProteomeXchange.

Title	Specific inhibition of splicing factor activity by decoy RNA oligonucleotides
Description	Alternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. We designed decoy oligonucleotides, composed of several repeats of a RNA motif, which is recognized by a single SF. Here we show that decoy oligonucleotides targeting splicing factors RBFOX1/2, SRSF1 and PTBP1, can specifically bind to their respective SFs and inhibit their splicing and biological activities both in vitro and in vivo. These decoy oligonucleotides present a novel approach to specifically downregulate SF activity and have the potential to treat diseases where SFs are up-regulated, such as cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:46.105.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Geiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-30 07:41:08	ID requested
1	2019-01-31 05:16:23	announced
2	2019-05-27 00:09:38	announced	Updated publication reference for PubMed record(s): 30962446.
⏵ 3	2024-10-22 04:52:48	announced	2024-10-22: Updated project metadata.

10.1038/s41467-019-09523-0;

Denichenko P, Mogilevsky M, Cl, é, ry A, Welte T, Biran J, Shimshon O, Barnabas GD, Danan-Gotthold M, Kumar S, Yavin E, Levanon EY, Allain FH, Geiger T, Levkowitz G, Karni R, Specific inhibition of splicing factor activity by decoy RNA oligonucleotides. Nat Commun, 10(1):1590(2019) [pubmed]

curator keyword: Biological

submitter keyword: Splicing factors,Alternative Splicing, RBFOX2, PTBP1, Cancer, lc-ms, SRSF1, Decoy oligonucleotides

Tamar Geiger
contact affiliation	Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
contact email	geiger@tauex.tau.ac.il
lab head
Tamar Geiger
contact affiliation	Weizmann Institute of Science
contact email	geiger@tauex.tau.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD012564

PRIDE project URI

• PRIDE
  1. PXD012564
     1. Label: PRIDE project
     2. Name: Specific inhibition of splicing factor activity by decoy RNA oligonucleotides