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PXD012557 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSmall RNA-mediated transgenerational silencing of histone genes impairs fertility in piRNA mutants
DescriptionPIWI-interacting RNAs (piRNAs) promote fertility in many animals. Yet, whether this is due to their conserved role in repressing repetitive elements (REs) or other functions remains unclear. Here, we show that the progressive loss of fertility in Caenorhabditis elegans lacking piRNAs is not caused by derepression of REs or other piRNA targets, but rather mediated by the epigenetic silencing of all the replicative histone genes. In the absence of piRNAs, downstream components of the piRNA pathway relocalize from germ granules and piRNA targets to histone mRNAs to synthesize antisense small RNAs (sRNAs) and induce transgenerational silencing. Removal of the downstream components of the piRNA pathway is sufficient to restore histone mRNA expression and fertility in piRNA mutants, and the inheritance of histone sRNAs in wild-type worms adversely affects their fertility for multiple generations. We conclude that the transgenerational silencing of histone genes contributes to the progressive loss of fertility in piRNA mutants and that coupling piRNAs and histone silencing may serve to maintain piRNAs production across evolution.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuillaume Arras
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-29 03:53:51ID requested
12019-12-11 06:03:32announced
22020-02-17 01:22:14announced2020-02-17: Updated publication reference for PubMed record(s): 32015436.
32021-07-19 22:52:10announced2020-02-17: Updated publication reference for PubMed record(s): 32015436.
2021-07-20: Updated publication reference for PubMed record(s): 32015436, 34108460.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: WAGO-1, Caenorhabditis elegans, LC-MS/MS
Contact List
Damarys Loew
contact affiliationLaboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University
contact emaildamarys.loew@curie.fr
lab head
Guillaume Arras
contact affiliationInstitut Curie
contact emailguillaume.arras@curie.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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