PXD012554-1

PXD012554 is an original dataset announced via ProteomeXchange.

Title	In situ imaging of the bacterial flagellar motor reveals stable inner- and outer-membrane associated sub-complexes during motor assembly and disassembly
Description	Using electron cryo-tomography (ECT), we show that, in three bacterial species, the flagellar motor disassembly process results in stable outer-membrane-embedded sub-complexes. These sub-complexes consist of the periplasmic embellished P- and L-rings, and bend the membrane inward while it remains apparently sealed. Our experiments show that these sub-complexes are more enriched in the cells at high optical-density (OD 600 ). In order to identify candidates for the protein(s) that may play a role in sealing the outer membrane we performed mass spectrometry experiments on membrane vesicles isolated from Shewanella oneidensis MR-1 cells grown to two different optical densities (OD 600 sample 1 ~ 5, OD 600 sample 2 ~ 0.6). Based on certain criteria, we identified few candidate proteins that are overexpressed in cells grown to high OD 600 and that might be involved in sealing the membrane after flagellar motor disassembly.
HostingRepository	PRIDE
AnnounceDate	2019-05-21
AnnouncementXML	Submission_2019-05-21_00:29:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Sweredoski
SpeciesList	scientific name: Shewanella oneidensis (strain MR-1); NCBI TaxID: 211586;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-29 03:31:50	ID requested
⏵ 1	2019-05-21 00:29:37	announced
2	2019-06-14 02:40:39	announced	Updated publication reference for PubMed record(s): 31109936.
3	2024-10-22 04:52:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Shewanella, flagellar motor

Spiros Garbis
contact affiliation	Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology
contact email	sgarbis@caltech.edu
lab head
Michael Sweredoski
contact affiliation	Proteome Exploration Laboratory
contact email	msweredo@caltech.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012554

PRIDE project URI

• PRIDE
  1. PXD012554
     1. Label: PRIDE project
     2. Name: In situ imaging of the bacterial flagellar motor reveals stable inner- and outer-membrane associated sub-complexes during motor assembly and disassembly