PXD012544-2

PXD012544 is an original dataset announced via ProteomeXchange.

Title	Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics
Description	Oligodendrocytes, the myelinating cells of the central nervous system, are essential for correct brain function. They originate from oligodendrocyte precursor cells (OPCs) through a differentiation process which is only incompletely understood and impaired in a variety of demyelinating diseases. Better knowledge of this differentiation holds the promise to develop novel therapies for these disorders. We investigated the differentiation of rat oligodendrocyte precursor cells to oligodendrocytes in vitro. After confirmation of differentiation by immunohistochemical analysis using cell type-specific marker proteins, we conducted a quantitative proteomics study using tandem mass tags (TMT). We investigated four time points of differentiation covering early, intermediate, and late stages. Data analysis was perfomed using Mascot and Maxquant.
HostingRepository	PRIDE
AnnounceDate	2019-06-21
AnnouncementXML	Submission_2019-06-25_06:25:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carmen Schoor
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-29 00:36:27	ID requested
1	2019-06-21 02:31:04	announced
⏵ 2	2019-06-25 06:25:30	announced	Updated publication reference for PubMed record(s): 31216117.
3	2024-10-22 04:52:48	announced	2024-10-22: Updated project metadata.

Schoor C, Brocke-Ahmadinejad N, Gieselmann V, Winter D, Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics. Proteomics, 19(14):e1900057(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mass spectrometry, Oligodendrocytes, Oligodendrocyte precursor cells, Quantification, TMT labeling

Dr. Dominic Winter
contact affiliation	Institute of Biochemistry and Molecular Biology (IBMB), University of Bonn
contact email	dominic.winter@uni-bonn.de
lab head
Carmen Schoor
contact affiliation	University Bonn
contact email	cschoor@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012544

PRIDE project URI

• PRIDE
  1. PXD012544
     1. Label: PRIDE project
     2. Name: Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics