PXD012523 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Secretome analysis of glioblastoma cells |
Description | PKR-like kinase (PERK) plays a significant role in inducing angiogenesis in various cancer-types including glioblastoma. Mass spectrometry screen on conditioned media from LN308 glioblastoma cell line treated with hypoxia revealed Peptidylglycine α-amidating monooxygenase (PAM) as a potential target inducing angiogenesis. PERK is found to be regulating PAM at mRNA level. PERK activation via CCT020312 (PERK activator) also increased the cleavage and thus the generation of sfCD domain of PAM in an oxygen-independent manner which acts as a signaling molecule from cytoplasm to the nuclei. PERK was found interacting with PAM in immuno-precipitation experiments suggesting a possible involvement in the generation of the PAM sfCD domain. Knockdown of PERK or PAM in LN308 cells reduced the formation of tubes by HUVECs in vitro. In vivo data also highlighted the importance of PAM in growth of glioblastoma where reduction of PAM expression in engrafted tumor significantly increased the survival in mice. In summary, PAM acts as a potential target for therapy against angiogenesis in glioblastoma. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-05 |
AnnouncementXML | Submission_2020-03-05_00:10:16.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD012523 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Chi Nguyen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Ammonia-loss; Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-28 01:20:52 | ID requested | |
⏵ 1 | 2020-03-05 00:25:24 | announced | |
Publication List
Soni H, Bode J, Nguyen CDL, Puccio L, Ne, ß, ling M, Piro RM, Bub J, Phillips E, Ahrends R, Eipper BA, Tews B, Goidts V, -amidating monooxygenase supports angiogenesis in glioblastoma. Oncogenesis, 9(2):18(2020) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: secretome, label-free, DDA, shot-gun proteomics, glioblastoma, PERK inhibitor |
Contact List
Robert Ahrends |
contact affiliation | Dr. Robert Ahrends Group leader lipidomics Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany |
contact email | robert.ahrends@isas.de |
lab head | |
Chi Nguyen |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Bunsen-Kirchhoff-Straße 11 | 44139 Dortmund, Germany |
contact email | chi.nguyen@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012523
- Label: PRIDE project
- Name: Secretome analysis of glioblastoma cells