PXD012522-2

PXD012522 is an original dataset announced via ProteomeXchange.

Title	Biopearling: Chains of interconnected outer membrane vesicles in a marine flavobacterium
Description	Large surface-to-volume ratios provide optimal nutrient uptake conditions for small microorganisms in oligotrophic habitats. The surface area can be increased with appendages. Here we describe chains of interconnecting vesicles protruding from cells of the flavobacterial strain Hel3_A1_48 originating from coastal free-living bacterioplankton. The chains were up to 10 µm long, had vesicles with a single membrane and a size of 80-100 nm by 50-80 nm, and emanated from the outer membrane. Cells extruded membrane tubes in the exponential phase, whereas vesicle chains dominated on cells in the stationary growth phase. This indicated a formation by pearling, which describes a physical morphogenic process: membrane tubes are protruding from liposomes and transform into chains of interconnected vesicles. Proteomes of whole cell membranes and of detached vesicles were dominated by outer membrane proteins including the type IX secretion system and surface-attached peptidases, glycoside hydrolases and endonucleases. Imported fluorescein-labeled laminarin was present in the periplasm of the cells and in the lumen of protruding vesicle chains. The appendages thus provide degradative enzymes on their surfaces and storage volume in the periplasmic extension, which seems to contribute to the high abundance of the Formosa-affiliated bacteria during laminarin utilization shortly after algal blooms.
HostingRepository	PRIDE
AnnounceDate	2019-07-25
AnnouncementXML	Submission_2019-07-29_00:59:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012522
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephanie Markert
SpeciesList	scientific name: Formosa sp. Hel3_A1_48; NCBI TaxID: 1336795;
ModificationList	Oxidation
Instrument	LTQ Orbitrap Classic

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-28 01:20:36	ID requested
1	2019-07-25 04:08:14	announced
⏵ 2	2019-07-29 00:59:47	announced	Updated publication reference for PubMed record(s): 31324630.

Fischer T, Schorb M, Reintjes G, Kolovou A, Santarella-Mellwig R, Markert S, Rhiel E, Littmann S, Becher D, Schweder T, Harder J, Biopearling of Interconnected Outer Membrane Vesicle Chains by a Marine Flavobacterium. Appl Environ Microbiol, 85(19):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Outer membrane vesicles, Biopearling, Flavobacteria, Formosa, algal blooms

Stephanie Markert
contact affiliation	Universität Greifswald Institut für Pharmazie, AG Pharmazeutische Biotechnologie Greifswald, Germany
contact email	stephanie.markert@uni-greifswald.de
lab head
Stephanie Markert
contact affiliation	University of Greifswald
contact email	stephanie.markert@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012522
     1. Label: PRIDE project
     2. Name: Biopearling: Chains of interconnected outer membrane vesicles in a marine flavobacterium