PXD012504 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology |
| Description | A new analytical method capable of simultaneously assessing cellular protein expression and cysteine oxidation would provide a valuable tool for the field of cysteine-targeted biology. Here, we show a workflow based on protein quantification using metabolic labeling and determination of cysteine oxidation using reporter ion quantification. We applied this approach to determine protein and redox changes in cells after 5-min, 60-min and 32-hours exposure to H2O2, respectively. Based on functional analysis of our data, we confirmed a biological relevance of this approach and its applicability for parallel mapping of cellular proteomes and redoxomes under diverse conditions. In addition, we revealed a specific pattern of redox changes in peroxiredoxins in a short time-interval cell exposure to H2O2. Overall, our present study offers an innovative, versatile experimental approach to multifaceted assessment of cellular proteome and its redox status, with broad implications for biomedical research towards better understanding of organismal physiology and diverse disease conditions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-04 |
| AnnouncementXML | Submission_2026-09-04_01:47:17.517.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Marie Vajrychova |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | 6x(13)C labeled L-lysine; 6x(13)C; 2-pyrrolidone-5-carboxylic acid (Glu); cysTMT6plex reporter+balance reagent cysteine disulfide; monohydroxylated residue; deamidated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2019-01-25 01:49:33 | ID requested | |
| ⏵ 1 | 2026-09-04 01:47:18 | announced | |
Publication List
| 10.1016/j.redox.2019.101227; |
| Vajrychova M, Salovska B, Pimkova K, Fabrik I, Tambor V, Kondelova A, Bartek J, Hodny Z, Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology. Redox Biol, 24():101227(2019) [pubmed] |
Keyword List
| curator keyword: Biological |
| submitter keyword: cysteine, liquid chromatography/mass spectrometry,SILAC-iodoTMT labeling, peroxiredoxin, proteome, redoxome |
Contact List
| Zdenek Hodny |
|---|
| contact affiliation | Department of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic |
| contact email | hodny@img.cas.cz |
| lab head | |
| Marie Vajrychova |
|---|
| contact affiliation | University Hospital Hradec Kralove |
| contact email | m.vajrychova@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012504
- Label: PRIDE project
- Name: Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology
