PXD012450-2

PXD012450 is an original dataset announced via ProteomeXchange.

Title	A post-transcriptional respiratome regulon in trypanosomes
Description	Post-transcriptional regulons coordinate the expression of groups of genes in eukaryotic cells, yet relatively few have been characterised. Parasitic trypanosomatids are particularly good models for studies on such mechanisms because they exhibit almost exclusive polycistronic, and unregulated, transcription. Here, we identify the Trypanosoma brucei ZC3H39/40 RNA-binding proteins as regulators of the respiratome; the mitochondrial electron transport chain (complexes I-IV) and the FoF1-ATP synthase (complex V). A high-throughput RNAi screen initially implicated both ZC3H proteins in variant surface glycoprotein (VSG) gene silencing. This link was confirmed and both proteins were shown to form a cytoplasmic ZC3H39/40 complex. Transcriptome and mRNA-interactome analyses indicated that the impact on VSG silencing was indirect, while the ZC3H39/40 complex specifically bound and stabilised transcripts encoding respiratome-complexes. Quantitative proteomic analyses revealed specific positive control of respiratome protein abundance. Our findings establish a link between the mitochondrial respiratome and VSG gene silencing. They also reveal a major respiratome regulon controlled by the conserved trypanosomatid ZC3H39/40 RNA-binding proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:15:22.136.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Trenaman
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-21 06:45:08	ID requested
1	2019-11-12 18:14:24	announced
⏵ 2	2024-10-22 04:15:23	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkz455;

Trenaman A, Glover L, Hutchinson S, Horn D, A post-transcriptional respiratome regulon in trypanosomes. Nucleic Acids Res, 47(13):7063-7077(2019) [pubmed]

curator keyword: Biological

submitter keyword: SILAC, ZC3H39/40,Trypanosoma brucei

Professor David Horn
contact affiliation	Biological Chemistry and Drug Discovery, Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee
contact email	dhorn@dundee.ac.uk
lab head
Anna Trenaman
contact affiliation	School of Life Sciences University of Dundee
contact email	atrenaman@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012450

PRIDE project URI

• PRIDE
  1. PXD012450
     1. Label: PRIDE project
     2. Name: A post-transcriptional respiratome regulon in trypanosomes