PXD012448-2

PXD012448 is an original dataset announced via ProteomeXchange.

Title	The Human Gut Firmicute Roseburia intestinalis is a Primary Degrader of Dietary β-Mannans
Description	β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2019-02-21
AnnouncementXML	Submission_2019-02-27_02:18:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Roseburia intestinalis L1-82; NCBI TaxID: 536231;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-21 06:44:13	ID requested
1	2019-02-21 07:09:38	announced
⏵ 2	2019-02-27 02:18:48	announced	Updated publication reference for PubMed record(s): 30796211.
3	2024-10-22 04:15:12	announced	2024-10-22: Updated project metadata.

La Rosa SL, Leth ML, Michalak L, Hansen ME, Pudlo NA, Glowacki R, Pereira G, Workman CT, Arntzen M Ø, Pope PB, Martens EC, Hachem MA, Westereng B, -mannans. Nat Commun, 10(1):905(2019) [pubmed]

curator keyword: Biological

submitter keyword: Q-Exactive, Roseburia intestinalis

Bjørge Westereng
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway
contact email	bjorge.westereng@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012448
     1. Label: PRIDE project
     2. Name: The Human Gut Firmicute Roseburia intestinalis is a Primary Degrader of Dietary β-Mannans