PXD012443-2

PXD012443 is an original dataset announced via ProteomeXchange.

Title	Nitrosoproteomic Analysis Deciphers the S-Nitrosylome in Carbon Metabolism of Tea Leaves
Description	Cysteine S-nitrosylation is a reversible protein post-translational modification and critically regulates the activity, localization and stability of proteins. Tea (Camellia sinensis (L.)) is one of the most thoroughly studied evergreen crop due to its broad non-alcoholic beverage and huge economic impact in the world. However, to date, little is known about the S-nitrosylome in this plant. Here, we performed a global analysis of cysteine S-nitrosylation in tea leaves. In total, 228 cysteine S-nitrosylation sites were identified in 191 proteins, representing the first extensive data on the S-nitrosylome in tea plants. These S-nitrosylated proteins were located in multiple subcellular compartments, especially in the chloroplast and cytoplasm. The analysis of functional enrichment and PPI network revealed that the S-nitrosylated proteins were mainly involved in carbon metabolism, especially in Calvin cycle and TCA cycle. These results suggested that S-nitrosylated proteins in tea leaves might play critical regulatory roles in the carbon metabolism. Overall, this study not only globally analyzed the functional annotation of cysteine S-nitrosylation in tea leaves, but also preliminarily provided the valuable information for further investigating the functions of cysteine S-nitrosylation in tea plants.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:07.506.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lihuan Qin
SpeciesList	scientific name: Camellia sinensis; NCBI TaxID: 4442;
ModificationList	nitrated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-21 06:41:39	ID requested
1	2020-05-26 13:14:05	announced
⏵ 2	2024-10-22 05:05:08	announced	2024-10-22: Updated project metadata.

Qiu C, Sun J, Wang Y, Sun L, Xie H, Ding Y, Qian W, Ding Z, First nitrosoproteomic profiling deciphers the cysteine S-nitrosylation involved in multiple metabolic pathways of tea leaves. Sci Rep, 9(1):17525(2019) [pubmed]

10.1038/s41598-019-54077-2;

curator keyword: Biological

submitter keyword: Cysteine S-nitrosylation,Camellia sinensis (L.) O. Kuntze, Carbon metabolism

Zhaotang Ding
contact affiliation	Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China
contact email	dzttea@163.com
lab head
lihuan Qin
contact affiliation	PTM Biolabs Inc.
contact email	lihuan_qin@ptm-biolab.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD012443

PRIDE project URI

• PRIDE
  1. PXD012443
     1. Label: PRIDE project
     2. Name: Nitrosoproteomic Analysis Deciphers the S-Nitrosylome in Carbon Metabolism of Tea Leaves