PXD012407-2

PXD012407 is an original dataset announced via ProteomeXchange.

Title	Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix
Description	By analyzing isolated collagen gel samples, we demonstrated in situ detection of spectrally deconvoluted auto-cathodoluminescence signatures of specific molecular content with precise spatial localization over a maximum field of view of 300 µm. Correlation of the secondary electron and the hyperspectral images proved ~40 nm resolution in the optical channel, obtained due to a short carrier diffusion length, suppressed by fibril dimensions and poor electrical conductivity specific to their organic composition. By correlating spectrally analyzed auto-cathodoluminescence with mass spectroscopy data, we differentiated spectral signatures of two extracellular matrices, namely human fibrin complex and rat tail collagen isolate, and uncovered differences in protein distributions of isolated extracellular matrix networks of heterogeneous populations. Furthermore, we demonstrated that cathodoluminescence can monitor the progress of a human cell-mediated remodeling process, where human collagenous matrix was deposited within a rat collagenous matrix. The revealed change of the heterogeneous biological composition was confirmed by mass spectroscopy.
HostingRepository	PRIDE
AnnounceDate	2019-02-19
AnnouncementXML	Submission_2019-02-27_02:17:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012407
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hans Mattias Larsson
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Phospho; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-17 03:12:53	ID requested
1	2019-02-19 04:29:56	announced
⏵ 2	2019-02-27 02:17:33	announced	Updated publication reference for PubMed record(s): 30793047.

Zielinski MS, Vardar E, Vythilingam G, Engelhardt EM, Hubbell JA, Frey P, Larsson HM, Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix. Commun Biol, 2():69(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Cathodoluminescence-SEM, Extracellular matrix

Hans Mattias Larsson
contact affiliation	Institute of bioengineering, School of Life science, EPFL, Switzerland
contact email	mattiashans.larsson@epfl.ch
lab head
Hans Mattias Larsson
contact affiliation	EPFL
contact email	mattiashans.larsson@epfl.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012407

PRIDE project URI

• PRIDE
  1. PXD012407
     1. Label: PRIDE project
     2. Name: Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix