PXD012377-2

PXD012377 is an original dataset announced via ProteomeXchange.

Title	A folded conformation of MukBEF and Cohesin
Description	Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the underlying conformational changes are largely unclear. Using structural biology, mass spectrometry and cross-linking, we investigated the architecture of two evolutionarily distant SMC-kleisin complexes: proteobacterial MukBEF and eukaryotic cohesin. We show that both contain a dynamic coiled-coil discontinuity, the elbow, near the middle of their arms that permits a folded conformation. Bending at the elbow brings into proximity the hinge dimerization domain and the head/kleisin module, situated at opposite ends of the arms. Our findings favor SMC activity models that include a large conformational change in the arms, such as a relative movement between DNA binding sites during DNA loading and translocation
HostingRepository	PRIDE
AnnounceDate	2019-03-01
AnnouncementXML	Submission_2019-05-27_01:40:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis O'Reilly
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	carbamoylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 06:25:37	ID requested
1	2019-03-01 00:07:50	announced
⏵ 2	2019-05-27 01:40:50	announced	Updated publication reference for PubMed record(s): 30833788.
3	2024-10-22 04:52:04	announced	2024-10-22: Updated project metadata.

B, ü, rmann F, Lee BG, Than T, Sinn L, O'Reilly FJ, Yatskevich S, Rappsilber J, Hu B, Nasmyth K, L, ö, we J, A folded conformation of MukBEF and cohesin. Nat Struct Mol Biol, 26(3):227-236(2019) [pubmed]

curator keyword: Biological

submitter keyword: crosslinking, MukBEF, cohesin, condensin, kleisin, Structural Maintenance of Chromosomes, SMC proteins, DNA translocation, loop extrusion

Juri Rappsilber
contact affiliation	Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK.
contact email	juri.rappsilber@ed.ac.uk
lab head
Francis O'Reilly
contact affiliation	TU Berlin
contact email	oreilly@tu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012377

PRIDE project URI

• PRIDE
  1. PXD012377
     1. Label: PRIDE project
     2. Name: A folded conformation of MukBEF and Cohesin