PXD012374-2

PXD012374 is an original dataset announced via ProteomeXchange.

Title	Analysis of the midbody interactome reveals new roles for PP1 phosphatases in late cytokinesis
Description	The midbody is an organelle assembled at the intercellular bridge connecting the two daughter cells at the end of mitosis. It is essential for the final separation of the daughter cells and involved in other important processes, including cell fate, pluripotency, apical-basal polarity, and cilium/lumen formation. The midbody is composed of numerous proteins with diverse functions distributed in a precise pattern. Here we report the first characterization of the midbody protein-protein interaction network (interactome), which provides an extremely valuable resource for understanding its multiple roles. Analysis of this midbody interactome led to the discovery that PP1/MYPT1 phosphatase regulates microtubule dynamics in late cytokinesis through de-phosphorylation of the kinesin MKLP1/KIF23, a finding that unexpectedly expands the temporal window of activity of this phosphatase during mitosis.
HostingRepository	PRIDE
AnnounceDate	2019-11-14
AnnouncementXML	Submission_2019-11-14_04:23:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 05:21:30	ID requested
1	2019-08-29 06:27:15	announced
⏵ 2	2019-11-14 04:24:07	announced	2019-11-14: Updated publication reference for PubMed record(s): 31586073.
3	2024-10-22 04:52:15	announced	2024-10-22: Updated project metadata.

Capalbo L, Bassi ZI, Geymonat M, Todesca S, Copoiu L, Enright AJ, Callaini G, Riparbelli MG, Yu L, Choudhary JS, Ferrero E, Wheatley S, Douglas ME, Mishima M, D'Avino PP, The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nat Commun, 10(1):4513(2019) [pubmed]

curator keyword: Biological

submitter keyword: Cytokinesis, SILAC, Maxquant, Obritrap, LC-MS/MS, midbody, interactome

Jyoti Choudhary
contact affiliation	Wellcome Trust Sanger Institute & The Institute of Cancer Research
contact email	jyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliation	Functional Proteomics, Institute Cancer Research & Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
contact email	jw13@sanger.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD012374

PRIDE project URI

• PRIDE
  1. PXD012374
     1. Label: PRIDE project
     2. Name: Analysis of the midbody interactome reveals new roles for PP1 phosphatases in late cytokinesis