PXD012373-3

PXD012373 is an original dataset announced via ProteomeXchange.

Title	Nuclear pore component Nup155 is part of the p53 network in liver cancer
Description	Cancer-relevant signalling pathways rely on bidirectional nucleocytoplasmic transport events through the nuclear pore complex (NPC). However, mechanisms by which individual NPC components (Nups) participate in the regulation of these pathways remain poorly understood. We discovered by integrating large scale proteomics, polysome fractionation and a focused RNAi approach that Nup155 controls mRNA translation of p21 (CDKN1A), a key mediator of the p53 response. The underlying mechanism involves transcriptional regulation of the putative tRNA and rRNA methyltransferase FTSJ1 by Nup155. Furthermore, we observed that Nup155 and FTSJ1 are p53 repression targets and accordingly found a correlation between the p53 status, Nup155 and FTSJ1 expression in murine and human hepatocellular carcinoma (HCC). Our data suggest an unanticipated regulatory network linking translational control by and repression of a structural NPC component modulating the p53 pathway through its effectors.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:16.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Ori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	4x(2)H labeled dimethylated L-lysine; acetylated residue; N6,N6-dimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 05:13:05	ID requested
1	2019-04-09 08:26:36	announced
2	2019-05-19 23:57:22	announced	Updated publication reference for PubMed record(s): 31089132.
⏵ 3	2024-10-22 04:52:25	announced	2024-10-22: Updated project metadata.

10.1038/s41467-019-10133-z;

Holzer K, Ori A, Cooke A, Dauch D, Drucker E, Riemenschneider P, Andres-Pons A, DiGuilio AL, Mackmull MT, Ba, ß, ler J, Roessler S, Breuhahn K, Zender L, Glavy JS, Dombrowski F, Hurt E, Schirmacher P, Beck M, Singer S, Nucleoporin Nup155 is part of the p53 network in liver cancer. Nat Commun, 10(1):2147(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: cancer,liver, nuclear pore, p53

Alessandro Ori
contact affiliation	Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), Jena, Germany
contact email	alessandro.ori@leibniz-fli.de
lab head
Alessandro Ori
contact affiliation	Leibniz Institute on Aging
contact email	aleori@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012373
     1. Label: PRIDE project
     2. Name: Nuclear pore component Nup155 is part of the p53 network in liver cancer