PXD012345-1

PXD012345 is an original dataset announced via ProteomeXchange.

Title	METAPROTEOMIC INVESTIGATION OF BIOAEROSOL FROM WORK ENVIRONMENTS
Description	Metaproteomic analysis of air particulate matter provides information about the properties of bioaerosols in the atmosphere and their influence on climate and public health. In this work, a new method for the extraction and analysis of proteins in airborne particulate matter from quartz microfiber filters was developed. Different protein extraction procedures were tested in order to select the best extraction protocol in terms of protein recovery. The optimized method was tested for extraction of proteins from spores of ubiquitous bacteria species and used for the first time for the metaproteomics characterization of filters from work environment. In particular, ambient aerosol samples were collected in different working environments, i.e. a composting plant, wastewater treatment plant and agricultural holding. One-hundred seventy-nine, 15, 205 and 444 proteins were successfully identified in composting plant, wastewater treatment plant, and agricultural holding, respectively. All identified proteins were mainly originate from fungi, bacteria and plants which is in line with the major categories of primary biological aerosol particles. The paper is the first metaproteomic study applied to bioaereosol samples collected in occupationally relevant environmental sites providing interesting information on the composting, wastewater treatment and feed blending processes. Significance This manuscript describes the metaproteomic analysis of aerosol samples collected in work enviroments. This is a novel use of aereosol samples and is needed as there is no really comprehensive way of analysing aereosol samples from a metaproteomic point of view. This paper could help to advance methods for metaproteomic analysis of bioaersols, specifically by comparing protein extraction protocols and pairing the best performing extraction protocol with a gel-free protein separation procedure applied for the first time for analysis of bioaerosol samples. The obtained data showed as bioaerosol was essentially made of fungi, bacteria and plant proteins, many of which could be associated to possible aerosolisation and could be a major health concern for workers on site and to the populations residing in neighbouring area.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:26:58.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Laura Capriotti
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-15 01:42:57	ID requested
⏵ 1	2025-07-13 16:26:59	announced

Macedo-Silva C, Albuquerque-Castro Â, Carri, ç, o I, Lencart J, Carneiro I, Altucci L, Lobo J, Miranda-Gon, ç, alves V, Henrique R, Correia MP, Jer, ó, nimo C, Decoding MUC1 and AR axis in a radiation-induced neuroendocrine prostate cancer cell-subpopulation unveils novel therapeutic targets. Cell Death Discov, 11(1):306(2025) [pubmed]

10.1038/s41420-025-02597-4;

curator keyword: Metaproteomics, Technical

submitter keyword: bioaereosol

metaproteomics

extraction methods

composting plant

wastewater treatment plant

agricultural holding

high resolution mass spectrometry

Anna Laura Capriotti
contact affiliation	Dipartimento di Chimica, Sapienza Università di Roma, Roma, Italy
contact email	annalaura.capriotti@uniroma1.it
lab head
Anna Laura Capriotti
contact affiliation	Sapienza University of Rome
contact email	annalaura.capriotti@uniroma1.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD012345

PRIDE project URI

• PRIDE
  1. PXD012345
     1. Label: PRIDE project
     2. Name: METAPROTEOMIC INVESTIGATION OF BIOAEROSOL FROM WORK ENVIRONMENTS