PXD012302-2

PXD012302 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Dpy19l2-deficient human globozoospermia reveals multiple molecular defects
Description	Globozoospermia is a severe teratozoospermia characterized by round-headed spermatozoa without acrosomes, resulting in male infertility. The clinical treatment of globozoospermia involves primarily intracytoplasmic sperm injection because of the low fertilization rate. The deletion of Dpy19l2 is known to be the major cause of globozoospermia. The defects in Dpy19l2-deficient human sperm at the protein level were characterized by performing a isotope-based quantitative proteomic analysis between Dpy19l2-deficient globozoospermic spermatozoa and normal controls. A total of 2,549 proteins were identified, including 495 differentially expressed proteins (fold-change >2), of which 322 were upregulated and 123 were downregulated. The levels of several important proteins, including SPACA 1, IZUMO1, ZPBP1, and PLCZ1, were decreased in globozoospermic sperm. Bioinformatics analysis indicated the Dpy19l2-deficient sperm presented molecular defects in acrosome, chromatin, sperm-egg interaction, and fertilization. This study may provide insights into the mechanism of globozoospermia and help develop better treatments to increase the success rate of fertilization.
HostingRepository	PRIDE
AnnounceDate	2019-08-15
AnnouncementXML	Submission_2019-08-22_01:36:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaofei Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-10 04:11:57	ID requested
1	2019-08-15 00:25:00	announced
⏵ 2	2019-08-22 01:44:53	announced	Updated publication reference for PubMed record(s): 31424156.
3	2024-10-22 04:52:23	announced	2024-10-22: Updated project metadata.

Guo Y, Jiang J, Zhang H, Wen Y, Zhang H, Cui Y, Tian J, Jiang M, Liu X, Wang G, Li Y, Hu Z, Zhou Z, Sha J, Chen D, Yang X, Guo X, Proteomic Analysis of Dpy19l2-Deficient Human Globozoospermia Reveals Multiple Molecular Defects. Proteomics Clin Appl, 13(6):e1900007(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: globozoospermia, DPY19L2, proteomics, round-headed, human

Xuejiang Guo
contact affiliation	Nanjing Med Univ, State Key Lab Reprod Med, Dept Histol & Embryol, 140 Hanzhong Rd, Nanjing 210029, Jiangsu, Peoples R China.
contact email	guo_xuejiang@njmu.edu.cn
lab head
Xiaofei Liu
contact affiliation	Nanjing Medical University
contact email	1215224830@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012302
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Dpy19l2-deficient human globozoospermia reveals multiple molecular defects