PXD012291-3

PXD012291 is an original dataset announced via ProteomeXchange.

Title	Shigella flexneri effectors IpaJ and VirA globally impair host conventional secretion
Description	Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work we report the multiple effects of two S. flexneri effectors, IpaJ and VirA, which target small GTPases of the Arf and Rab families, consequently inhibiting several intracellular trafficking pathways. IpaJ and VirA induce large-scale impairment of host protein secretion and block the recycling of surface receptors. Moreover, these two effectors decrease clathrin-dependent and -independent endocytosis. Therefore, S. flexneri infection induces a global blockage of host cell intracellular transport, affecting the exchange between cells and their external environment. The combined action of these effectors disorganizes the epithelial cell polarity, disturbs epithelial barrier integrity, promotes multiple invasion events and enhances the pathogen capacity to penetrate into the colonic tissue in vivo.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:06.357.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Arras
SpeciesList	scientific name: Shigella flexneri; NCBI TaxID: 623;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-09 07:46:38	ID requested
1	2019-06-04 07:12:25	announced
2	2019-06-20 06:57:55	announced	Updated publication reference for PubMed record(s): 31209035.
⏵ 3	2024-10-22 04:51:07	announced	2024-10-22: Updated project metadata.

Ferrari ML, Malard, é V, Grassart A, Salavessa L, Nigro G, Descorps-Declere S, Rohde JR, Schnupf P, Masson V, Arras G, Loew D, Sansonetti PJ, Sauvonnet N, promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport. Proc Natl Acad Sci U S A, 116(27):13582-13591(2019) [pubmed]

10.1073/pnas.1902922116;

curator keyword: Biological

submitter keyword: secretion, IpaJ, SILAC,Bacteria, VirA, pathogen, polarity

Damarys Loew
contact affiliation	Laboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University
contact email	Damarys.Loew@curie.fr
lab head
Guillaume Arras
contact affiliation	Institut Curie
contact email	guillaume.arras@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012291

PRIDE project URI

• PRIDE
  1. PXD012291
     1. Label: PRIDE project
     2. Name: Shigella flexneri effectors IpaJ and VirA globally impair host conventional secretion