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PXD012227-1

PXD012227 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhDHS is involved in chloroplast development in petunia
DescriptionDeoxyhypusine synthase (DHS) is encoded by a nuclear gene and is the key enzyme involved in the post-translational activation of the eukaryotic translation initiation factor eIF5A. DHS plays important roles in plant growth and development. To gain a better understanding of DHS, the petunia (Petunia hybrida) PhDHS gene was isolated, and the role of PhDHS in plant growth was analysed. PhDHS protein was localized to the nucleus and cytoplasm. Virus-mediated PhDHS silencing caused a sectored chlorotic leaf phenotype. The chlorophyll levels was reducted and the development of chloroplasts was abnormal in PhDHS-silenced leaves These features were not observed in DHS-suppressed Arabidopsis thaliana and Solanum lycopersicum. These results indicated that PhDHS is required for development in petunia. A proteome assay showed that 308 proteins are up-regulated and 266 proteins are down-regulated in PhDHS-silenced plants compared with control. Among them, 20 proteins in photosystem I and photosystem II and 11 thylakoid proteins were down-regulated in PhDHS-silenced leaves, further supporting the involvement of PhDHS in photosynthesis in petunia.
HostingRepositoryPRIDE
AnnounceDate2019-11-13
AnnouncementXMLSubmission_2019-11-12_16:57:11.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxiaomei li
SpeciesList scientific name: Petunia; NCBI TaxID: 4101;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-03 03:52:37ID requested
12019-11-12 16:57:12announced
Publication List
Liu J, Chang X, Ding B, Zhong S, Peng L, Wei Q, Meng J, Yu Y, PhDHS Is Involved in Chloroplast Development in Petunia. Front Plant Sci, 10():284(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Deoxyhypusine synthase, Chloroplast development, sectored chlorotic leaf, Photosynthesis, Petunia
Contact List
Yixun Yu
contact affiliationGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact emailyuyixun@scau.edu.cn
lab head
xiaomei li
contact affiliationPTM Biolabs lnc.
contact emailxiaomei_li@ptm-biolab.com
dataset submitter
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Dataset FTP location
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