PXD012193 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Glomerular filtrate proteins in acute cardiorenal syndrome |
Description | Acute cardiorenal syndrome (CRS-1) is a morbid complication of acute cardiovascular disease. Mechanistic investigations have focused on intrarenal cellular signaling induced by ischemia/reperfusion. Additional cardiorenal connector signals have been postulated, but investigation in CRS-1 has been limited by a paucity of animal models and technical limitations precluding discovery studies of glomerular filtrate. To address these limitations, we developed a translational model of CRS-1, cardiac arrest and cardiopulmonary resuscitation (CA/CPR) in mice. Quantitative proteomics using isobaric tagging (10-plex TMT reagents, Thermo Fisher) was performed on 24h urine collections from mice with deficient tubular endocytosis and from littermate controls, before and after CA/CPR. Data acquisition used the SPS MS3 method on a Thermo Fusion Tribrid mass spectrometer for accurate reporter ion signals. The findings confirmed CA/CPR-specific cardiac proteins in urine and identified a novel CA/CPR-specific filtrate component: Cardiac LIM protein (CSRP3). |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-27 |
AnnouncementXML | Submission_2019-03-26_05:46:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Phillip Wilmarth |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-24 05:55:55 | ID requested | |
1 | 2019-02-27 06:47:35 | announced | |
⏵ 2 | 2019-03-26 05:46:39 | announced | Updated publication reference for PubMed record(s): 30829647. |
Publication List
Wakasaki R, Matsushita K, Golgotiu K, Anderson S, Eiwaz MB, Orton DJ, Han SJ, Lee HT, Smith RD, Rodland KD, Piehowski PD, Hutchens MP, Glomerular filtrate proteins in acute cardiorenal syndrome. JCI Insight, 4(4):(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Mouse model, cardiovascular disease, acute cardiorenal syndrome, urine, quantitative proteomics, isobaric labeling, tandem mass tags |
Contact List
Dr. Michael P. Hutchens |
contact affiliation | Anesthesiology & Perioperative Medicine Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA |
contact email | hutchenm@ohsu.edu |
lab head | |
Phillip Wilmarth |
contact affiliation | OHSU |
contact email | wilmarth@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012193
- Label: PRIDE project
- Name: Glomerular filtrate proteins in acute cardiorenal syndrome