PXD012192 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peptidomics analysis of milk protein-derived peptides released over time in the preterm infant stomach |
Description | Over the course of milk digestion, native milk proteases and infant digestive proteases fragment intact proteins into peptides with potential bioactivity. This study investigated the release of peptides over three hours of gastric digestion in 14 preterm infant sample sets. The peptide content was extracted and analyzed from milk and gastric samples via Orbitrap tandem mass spectrometry. The relative ion intensity (abundance) and count of peptides in each sample were compared over time and between infants fed milk fortified with bovine milk fortifier and infants fed unfortified milk. Bioactivity of the identified peptides was predicted by sequence homology to known bioactive milk peptides. Both total and bioactive peptide abundance and count continuously increased over three hours of gastric digestion. After accounting for infant weight, length, and post-conceptual age, fortification of milk limited the release of peptides from human milk proteins. Peptides that survived further gastric digestion after their initial release were structurally more similar to bioactive peptides than non-surviving peptides. This work is the first to provide a comprehensive profile of milk peptides released during gastric digestion over time, which is an essential step in determining which peptides are most likely to be biologically relevant in the infant. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-12 |
AnnouncementXML | Submission_2019-03-11_21:56:50.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Beverly |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-24 05:53:58 | ID requested | |
⏵ 1 | 2019-03-11 21:56:51 | announced | |
Publication List
Beverly RL, Underwood MA, Dallas DC, Peptidomics Analysis of Milk Protein-Derived Peptides Released over Time in the Preterm Infant Stomach. J Proteome Res, 18(3):912-922(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Peptide, Human milk, Gastric, Preterm, Bioactive, Digestion |
Contact List
David Dallas |
contact affiliation | Nutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University |
contact email | dave.dallas@oregonstate.edu |
lab head | |
Robert Beverly |
contact affiliation | Oregon State University |
contact email | beverlyr@oregonstate.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012192
- Label: PRIDE project
- Name: Peptidomics analysis of milk protein-derived peptides released over time in the preterm infant stomach