PXD012143-1

PXD012143 is an original dataset announced via ProteomeXchange.

Title	Cyclic AMP signalling controls key components of malaria parasite host cell invasion machinery
Description	Cyclic nucleotides are important signalling molecules across evolution, but their roles in malaria parasites are poorly understood. We have investigated the role of cAMP in asexual blood stage development of Plasmodium falciparum through conditional disruption of adenylyl cyclase (ACβ) and its downstream effector, cAMP-dependent protein kinase (PKA). We show that both production of cAMP and activity of PKA are critical for erythrocyte invasion, whilst key developmental steps that precede invasion still take place in the absence of cAMP-dependent signalling. We also show that another protein with putative cyclic nucleotide binding sites, PfEpac, does not play an essential role in cyclic nucleotide signalling in blood stages. We identify over 2,000 sites whose phosphorylation is dependent on cAMP signalling and we provide mechanistic insight as to how cAMP-dependent phosphorylation of the cytoplasmic domain of the essential invasion adhesin apical membrane antigen 1 (AMA1) controls erythrocyte invasion.
HostingRepository	PRIDE
AnnounceDate	2019-05-14
AnnouncementXML	Submission_2019-05-14_06:50:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helen Flynn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-21 01:39:15	ID requested
⏵ 1	2019-05-14 06:50:48	announced
2	2024-10-22 04:50:58	announced	2024-10-22: Updated project metadata.

Patel A, Perrin AJ, Flynn HR, Bisson C, Withers-Martinez C, Treeck M, Flueck C, Nicastro G, Martin SR, Ramos A, Gilberger TW, Snijders AP, Blackman MJ, Baker DA, Cyclic AMP signalling controls key components of malaria parasite host cell invasion machinery. PLoS Biol, 17(5):e3000264(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: cAMP‐dependent protein kinase, adenylyl cyclase β, cyclic nucleotide signalling, AMA1, host cell invasion, Plasmodium falciparum, malaria, Orbitrap Fusion Lumos

Ambrosius Snijders
contact affiliation	Head of Mass Spectrometry Proteomics The Francis Crick Institute 1 Midland Road London NW1 1AT
contact email	bram.snijders@crick.ac.uk
lab head
Helen Flynn
contact affiliation	The Francis Crick Institute
contact email	helen.flynn@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012143

PRIDE project URI

• PRIDE
  1. PXD012143
     1. Label: PRIDE project
     2. Name: Cyclic AMP signalling controls key components of malaria parasite host cell invasion machinery