PXD012084 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantifying Positional Isomers (QPI) by top-down mass spectrometry |
Description | Proteomics has exposed a plethora of post-translational modifications, but demonstrating functional relevance requires new approaches. Top-down proteomics can characterize co-occurring modifications in terms of localization, abundance and hierarchy. Here, we present a top-down MS analysis workflow for the discovery and quantification of proteoforms. Confident fragment assignment allows for localization of modification sites and quantification of all proteoforms, including positional isomers, as validated by investigating synthetic isoforms of ubiquitin and hyper-phosphorylated Bora. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-18 |
AnnouncementXML | Submission_2021-03-18_01:54:12.368.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Richard Scheltema |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-17 07:12:55 | ID requested | |
⏵ 1 | 2021-03-18 01:54:13 | announced | |
2 | 2023-05-14 14:16:05 | announced | 2023-05-14: Updated project metadata. |
Publication List
Brunner AM, L, รถ, ssl P, Geurink PP, Ovaa H, Albanese P, Altelaar AFM, Heck AJR, Scheltema RA, Quantifying Positional Isomers (QPI) by Top-Down Mass Spectrometry. Mol Cell Proteomics, 20():100070(2021) [pubmed] |
Keyword List
submitter keyword: Top-down mass spectrometry, positional isomers, phosphorylation, quantitation |
Contact List
Richard Scheltema |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences Padualaan 8 3584 CH Utrecht Utrecht University The Netherlands |
contact email | r.a.scheltema@uu.nl |
lab head | |
Richard Scheltema |
contact affiliation | Utrecht University |
contact email | r.a.scheltema@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012084
- Label: PRIDE project
- Name: Quantifying Positional Isomers (QPI) by top-down mass spectrometry