PXD012078 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Substrates of human Sec62/Sec63 complex |
Description | The Sec61-associated Sec62/Sec63 complex supports translocation of only a subset of precursor polypeptides, i.e. in a substrate-specific manner. To characterize Sec62/Sec63-dependent precursors, we combined siRNA-mediated Sec62 or Sec63 depletion in Hela cells or CRISPR/Cas9-mediated knock-down in HEK 293 cells with label-free quantitative proteomics and differential expression analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-06 |
AnnouncementXML | Submission_2020-03-06_04:14:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Richard Zimmermann |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-17 04:01:39 | ID requested | |
⏵ 1 | 2020-03-06 04:14:58 | announced | |
Publication List
Schorr S, Nguyen D, Ha, ß, denteufel S, Nagaraj N, Cavali, é A, Greiner M, Weissgerber P, Loi M, Paton AW, Paton JC, Molinari M, F, ö, rster F, Dudek J, Lang S, Helms V, Zimmermann R, Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import. FEBS J, 287(21):4612-4640(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: HeLa, HEK 293, ER protein import |
Contact List
Prof. Dr. Richard Zimmermann (lab head) |
contact affiliation | Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, Kirrbergerstr. 100, 66421 Homburg, Deutschland, fone +49 (0)6841 1626510, fax +49 (0)6841 1626288 Nagarjuna Nagaraj, Max Planck Institute for Biochemistry, Core facility (contact) |
contact email | richard.zimmermann@uks.eu |
lab head | |
Richard Zimmermann |
contact affiliation | Saarland University |
contact email | richard.zimmermann@uks.eu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012078 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012078
- Label: PRIDE project
- Name: Substrates of human Sec62/Sec63 complex