PXD012069-2

PXD012069 is an original dataset announced via ProteomeXchange.

Title	The FGF4-dependent phosphoproteome in mouse embryonic stem cells
Description	Protein kinase signalling is a major mechanism by which embryonic stem cell pluripotency and differentiation is controlled. However, the pathways and components that regulate embryonic stem cell identity have not been systematically defined. Here, we employ FGF4 signalling as a model system to investigate phosphoproteome dynamics in differentiating mouse embryonic stem cells. We report identification and quantitation of more than 10,000 phosphopeptides, of which hundreds of phosphophoylation sites are regulated more than 2-fold by acute FGF4 stimulation. We hypothesise that phosphorylation sites in this dataset are relevant for regulating the transition of mouse embryonic stem cells from pluripotency towards lineage specific differentiation.
HostingRepository	PRIDE
AnnounceDate	2020-02-24
AnnouncementXML	Submission_2020-05-18_06:52:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Houjiang Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-14 05:04:11	ID requested
1	2020-02-24 00:13:05	announced
⏵ 2	2020-05-18 06:52:35	announced	2020-05-18: Updated publication reference for PubMed record(s): 32170114.
3	2024-10-22 03:59:53	announced	2024-10-22: Updated project metadata.

Fernandez-Alonso R, Bustos F, Budzyk M, Kumar P, Helbig AO, Hukelmann J, Lamond AI, Lanner F, Zhou H, Petsalaki E, Findlay GM, Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation. Nat Commun, 11(1):1357(2020) [pubmed]

curator keyword: Biological

submitter keyword: Kinase, phosphorylation, growth factor, mouse embryonic stem cell

Greg Findlay
contact affiliation	The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, The University of Dundee, Dundee DD1 5EH, UK
contact email	g.m.findlay@dundee.ac.uk
lab head
Houjiang Zhou
contact affiliation	University of Dundee
contact email	h.y.zhou@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD012069

PRIDE project URI

• PRIDE
  1. PXD012069
     1. Label: PRIDE project
     2. Name: The FGF4-dependent phosphoproteome in mouse embryonic stem cells