PXD012061 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of the composition and interaction partners of the Nucleosome Remodeling and Deacetylase (NuRD) complex in cancer cells |
Description | The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for normal development in metazoans and has direct implications in a range of human diseases. Amongst all classes of chromatin remodeling complexes, the NuRD complex has been the most recalcitrant to analysis. This is, in part, due to difficulties with producing the intact complex recombinantly. Using affinity-purification followed by mass spectrometry, we determined the stoichiometry of the NuRD complex from three cancer cell lines representing three different human tissues. We also analysed the data to determine the interactome of the NuRD complex and identified potential NuRD interactors. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-13 |
AnnouncementXML | Submission_2019-03-13_14:26:54.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jason Low |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-14 01:54:08 | ID requested | |
⏵ 1 | 2019-03-13 14:26:55 | announced | |
Publication List
Sharifi Tabar M, Mackay JP, Low JKK, The stoichiometry and interactome of the Nucleosome Remodeling and Deacetylase (NuRD) complex are conserved across multiple cell lines. FEBS J, 286(11):2043-2061(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: NuRD, stoichiometry, iBAQ, interactome, shotgun proteomics |
Contact List
Joel Mackay |
contact affiliation | School of Life and Environmental Sciences, University of Sydney, Australia |
contact email | joel.mackay@sydney.edu.au |
lab head | |
Jason Low |
contact affiliation | The University of Sydney |
contact email | jason.low@sydney.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012061 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012061
- Label: PRIDE project
- Name: Comparison of the composition and interaction partners of the Nucleosome Remodeling and Deacetylase (NuRD) complex in cancer cells