PXD012054-1
PXD012054 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | High throughput generation of a resource of the human secretome in mammalian cells |
Description | The proteins secreted by human tissues (the secretome) are important for the basic understanding of human biology, but also for identification of potential targets for future diagnosis and therapy. Here, we present an annotation of all predicted secreted proteins (n=2,623) with information about their spatial distribution in the human body. A high-throughput mammalian cell factory was established to create a resource of recombinant full-length proteins. This resource was used for phenotypic assays involving β-cell dedifferentiation and for development of targeted proteomics assays. A comparison between host cells, including omics analysis, shows that many of the proteins that failed to be generated in CHO cells could be rescued in human HEK 293 cells. In conclusion, the human secretome has been mapped and characterized to facilitate further exploration of the human secretome. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-03-15 |
AnnouncementXML | Submission_2021-03-15_07:38:23.423.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | supported by repository but incomplete data and/or metadata |
PrimarySubmitter | Andreas Hober |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4) |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-12-13 16:36:01 | ID requested | |
⏵ 1 | 2021-03-15 07:38:24 | announced |
Publication List
Tegel H, Dannemeyer M, Kanje S, Sivertsson Å, Berling A, Svensson AS, Hober A, Enstedt H, Volk AL, Lundqvist M, Moradi M, Afshari D, Ekblad S, Xu L, Westin M, Bidad F, Schiavone LH, Davies R, Mayr LM, Knight S, G, ö, pel SO, Voldborg BG, Edfors F, Forsstr, ö, m B, von Feilitzen K, Zwahlen M, Rockberg J, Takanen JO, Uhl, é, n M, Hober S, High throughput generation of a resource of the human secretome in mammalian cells. N Biotechnol, 58():45-54(2020) [pubmed] |
Keyword List
submitter keyword: dia, human secretome, assay development, plasma |
Contact List
Bjorn Forsstrom | |
---|---|
contact affiliation | Science for Life Laboratory, KTH - Royal Institute of Technology |
contact email | bjorn.forsstrom@scilifelab.se |
lab head | |
Andreas Hober | |
contact affiliation | Science for Life Laboratory, KTH - Royal Institute of Technology |
contact email | andreas.hober@scilifelab.se |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |