PXD012053-2

PXD012053 is an original dataset announced via ProteomeXchange.

Title	Impact of Acute methionine restriction on proteome of Th1 cells - Part 2
Description	CD4 T cells were activated using CD3/CD28 antibodies in the presence of IL2 and IL12, to generate Th1 cells. Th1 cells were maintained and expanded in IL2 and IL12, on D5 Th1 cells were sorted for CD4+ expression and DAP1 exclusion. After flow sorting, live TH1 cells were resuspended (1e6 per ml) in methionine free RPMI, supplemented with 10% dialysed FBS, IL2 and IL12, and with L-methionine (100μM, or 1μM). Cells were cultured for 5 hrs before collection for proteomics processing.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:00:13.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-13 08:45:25	ID requested
1	2019-03-29 01:51:42	announced
⏵ 2	2024-10-22 04:00:14	announced	2024-10-22: Updated project metadata.

10.7554/elife.44210;

Sinclair LV, Howden AJ, Brenes A, Spinelli L, Hukelmann JL, Macintyre AN, Liu X, Thomson S, Taylor PM, Rathmell JC, Locasale JW, Lamond AI, Cantrell DA, Antigen receptor control of methionine metabolism in T cells. Elife, 8():(2019) [pubmed]

submitter keyword: metabolism, methioine, transport,T cells

Professor Doreen Cantrell
contact affiliation	Division of Cell Signalling and Immunology School of Life Sciences University of Dundee Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012053

PRIDE project URI

• PRIDE
  1. PXD012053
     1. Label: PRIDE project
     2. Name: Impact of Acute methionine restriction on proteome of Th1 cells - Part 2