PXD012014-1

PXD012014 is an original dataset announced via ProteomeXchange.

Title	A Novel Nutrient Deprivation-Induced Neonicotinoid Insecticide Acetamiprid Degradation by Ensifer adhaerens CGMCC 6315
Description	Proteomic Analysis. The proteomic expression of CGMCC 6315 under different nutrient concentration conditions was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics. The CGMCC 6315 was cultured in LB broth and 1/15LB broth as described above, after which the strains were collected by centrifugation at 10,000× g for 10 min at 4°C. Protein extraction, digestion, iTRAQ labeling and peptide fractionation were performed using the protocol described by Jin et al. 29. Protein identification was conducted using a LC-20AD nano-HPLC instrument (Shimadzu, Kyoto, Japan) equipped with a Q EXACTIVE tandem mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) for data-dependent acquisition detection by nano-electrospray ionization. The raw MS/MS data were converted into MGF format by the thermo scientific tool Proteome Discoverer, and the exported MGF files were searched using Mascot (version 2.3.02) against the selected database containing 7546 CGMCC 6315 coding genes. The IQuant software was used for quantitative analysis of the labeled peptides with isobaric tags. Fold changes of >1.7 with p-values <0.05 were used as a cut off for differentially regulated proteins.
HostingRepository	PRIDE
AnnounceDate	2018-12-12
AnnouncementXML	Submission_2018-12-12_03:22:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sun Shi-Lei
SpeciesList	scientific name: Ensifer adhaerens; NCBI TaxID: 106592;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 08:11:29	ID requested
⏵ 1	2018-12-12 03:22:26	announced
2	2019-06-17 05:20:34	announced	Updated publication reference for PubMed record(s): 30576131.
3	2024-10-22 03:58:50	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: iTRAQ

Shi-Lei Sun
contact affiliation	Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, Nanjing 210023, People’s Republic of China.
contact email	1159290841@qq.com
lab head
sun Shi-Lei
contact affiliation	Nanjing normal university
contact email	1159290841@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012014
     1. Label: PRIDE project
     2. Name: A Novel Nutrient Deprivation-Induced Neonicotinoid Insecticide Acetamiprid Degradation by Ensifer adhaerens CGMCC 6315