PXD012006-2

PXD012006 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome Dataset for Adult-derived Mouse Microglia
Description	We report methods development for quantitative proteomic analysis of samples with limited number of cells. The improvements include suspension trapping (S-Trap) for sample processing, longer analytical column length (75cm), and a segmented data dependent acquisition approach. The modified approach was applied to the proteomic analysis of adult-derived mouse microglia and optimized to the cell count expected from mouse brain (~300K microglial cells). With the improved approach, over 4500 quantifiable proteins can be obtained (in biological triplicate) with an average CV of 10%.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:29.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stanley Stevens
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 01:49:34	ID requested
1	2019-04-18 02:06:37	announced
⏵ 2	2024-10-22 03:58:37	announced	2024-10-22: Updated project metadata.

Publication List

10.1002/pmic.201800469;
Guergues J, Zhang P, Liu B, Stevens SM, Improved Methodology for Sensitive and Rapid Quantitative Proteomic Analysis of Adult-Derived Mouse Microglia: Application to a Novel In Vitro Mouse Microglial Cell Model. Proteomics, 19(11):e1800469(2019) [pubmed]

10.1002/pmic.201800469;

Guergues J, Zhang P, Liu B, Stevens SM, Improved Methodology for Sensitive and Rapid Quantitative Proteomic Analysis of Adult-Derived Mouse Microglia: Application to a Novel In Vitro Mouse Microglial Cell Model. Proteomics, 19(11):e1800469(2019) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: neuroinflammation,IMG cells, proteomics, mass spectrometry, microglia, dataset brief, glia

curator keyword: Biomedical

submitter keyword: neuroinflammation,IMG cells, proteomics, mass spectrometry, microglia, dataset brief, glia

Contact List

Stanley M. Stevens, Jr.
contact affiliation	Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, Colchester, VT 05446
contact email	stanley.stevens@acphs.edu
lab head
Stanley Stevens
contact affiliation	Albany College of Pharmacy and Health Sciences
contact email	stanley.stevens@acphs.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012006
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012006

PRIDE project URI

Repository Record List

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