PXD011995-4

PXD011995 is an original dataset announced via ProteomeXchange.

Title	Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC
Description	In this study, NAC was UV-crosslinked using the photo-crosslinking amino acid p-benzoyl-L-phenylalanine (pBPA) in order to determine specific interaction sites of the alphaNAC N-terminus in the native protein structure. Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-XL-MS) was used to compare crosslink abundances of a NAC mutant versus wild type NAC in order to determine the influence of the mutated motive on the structural dynamics of NAC.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:13.910.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-10 06:22:06	ID requested
1	2019-07-25 04:22:03	announced
2	2019-07-26 00:30:20	announced	Updated project metadata.
3	2019-08-16 00:26:00	announced	Updated project metadata.
⏵ 4	2024-10-22 04:51:17	announced	2024-10-22: Updated project metadata.

10.1016/j.molcel.2019.06.030;

Gamerdinger M, Kobayashi K, Wallisch A, Kreft SG, Sailer C, Schl, ö, mer R, Sachs N, Jomaa A, Stengel F, Ban N, Deuerling E, Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC. Mol Cell, 75(5):996-1006.e8(2019) [pubmed]

curator keyword: Technical

submitter keyword: NAC, structural mass spectrometry, UV-crosslinking, q-XL-MS

Florian Stengel
contact affiliation	Department of Biology, University of Konstanz, Germany
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011995

PRIDE project URI

• PRIDE
  1. PXD011995
     1. Label: PRIDE project
     2. Name: Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC