<<< Full experiment listing


PXD011995 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEarly Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC
DescriptionIn this study, NAC was UV-crosslinked using the photo-crosslinking amino acid p-benzoyl-L-phenylalanine (pBPA) in order to determine specific interaction sites of the alphaNAC N-terminus in the native protein structure. Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-XL-MS) was used to compare crosslink abundances of a NAC mutant versus wild type NAC in order to determine the influence of the mutated motive on the structural dynamics of NAC.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian Stengel
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-10 06:22:06ID requested
12019-07-25 04:22:03announced
22019-07-26 00:30:20announcedUpdated project metadata.
32019-08-16 00:26:00announcedUpdated project metadata.
Publication List
Gamerdinger M, Kobayashi K, Wallisch A, Kreft SG, Sailer C, Schl, ö, mer R, Sachs N, Jomaa A, Stengel F, Ban N, Deuerling E, Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC. Mol Cell, 75(5):996-1006.e8(2019) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: NAC, structural mass spectrometry, q-XL-MS, UV-crosslinking
Contact List
Florian Stengel
contact affiliationDepartment of Biology, University of Konstanz, Germany
contact emailflorian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliationUniversität Konstanz
contact emailflorian.stengel@uni-konstanz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011995
PRIDE project URI
Repository Record List
[ + ]