PXD011945-1

PXD011945 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Xcc Pan Proteome
Description	In this project, two isolates of Xanthomonas campestris pv campestris were compared in the following conditions, 1. growth in NYG rich medium, and 2. growth in XVM1 minimal medium. Total proteins were extracted and trypsin digested. The nanoscale Liquid Chromatography was performed to separate tryptic peptides in a nanoAcquity system (Waters, USA) using 2D dilution technology. The digested peptides were analyzed in a Synapt G2 HDMS mass spectrometer (Waters, Manchester, UK) operated in the resolution mode of analysis and a resolving power of at least 20,000 full-width half-maximum (FWHM). All analyses were performed using a positive nanoelectrospray ion mode (nanoESI +). ProteinLynx Global Server (PLGS) version 2.5 (Waters, Manchester, UK) was used to process the MS data obtained from LC-MSE. For protein identification, the software embedded ion accounting algorithm was used. For the analysis of protein identification and quantification level, the observed intensity measurements were auto normalized by PLGS.
HostingRepository	MassIVE
AnnounceDate	2018-12-07
AnnouncementXML	Submission_2018-12-07_03:36:11.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fabiano Touzdjian
SpeciesList	scientific name: Xanthomonas campestris pv. campestris; NCBI TaxID: 340;
ModificationList	Carbamyl; Oxidation
Instrument	Synapt G2 HDMS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-05 11:16:26	ID requested
⏵ 1	2018-12-07 03:36:12	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Shotgun proteomics, Xcc, Pan Proteome,

submitter keyword: Shotgun proteomics, Xcc, Pan Proteome,

Contact List

Angela Mehta
contact affiliation	Embrapa
contact email	angela.mehta@embrapa.br
lab head
Fabiano Touzdjian
contact affiliation	embrapa
contact email	fabianotouz@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083181

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083181